We hope you find this resource helpful.  If you would like to see additional topics included, please let us know. We encourage you to call our Technical Staff at 1-800-441-7508 to assist you in resolving any HPLC problems that you might encounter.

1.	What's Wrong with my HPLC Column - A Quick Reference
2.	Steps for Ensuring Good Column Performance
		Step 1: Prevent the Three Most Common Causes of Column Failure:     Loss of Bonded-Phase     Column Voiding     Column Contamination Step 2: Treat Your Reversed-Phase Column Well Step 3: Know When to Use and Change a Guard Column Step 4: Quickly Determine When a Column is Going Bad
3.	What is the Best Column Configuration for Your Method?
		Will a Shorter Column Work for Your Application? Will a Smaller-Bore Column Work for Your Application? Solvent Consumption is Directly Related to Column Volume Optimum Efficiency with Small-Bore Columns is Directly Related to Peak Volume Small-Bore Columns Require Reduced Flow Rates
4.	Extra-Column Volume
		Sources of Excessive Extra-Column Volume Determining if Excessive System Extra-Column Volume Exists Correcting Excessive Extra-Column Volume Effect of Extra-Column Volume on Small Bore Columns Effect of Extra-Column Volume on Highly Efficient Columns
5.	Column-to-Column Selectivity Changes - A Quick Diagnostic
		Effects of Column Selectivity Changes Causes of Column Selectivity Changes Classifying Selectivity Changes Correcting Selectivity Changes
6.	Injection-to-Injection Reproducibility
		Retention and Resolution Variability Identifying and Correcting Variability in Column Performance Retention Time Variability in Gradient Separations Causes and Solutions
7.	Last Resort Procedures to Restore Column Performance
		How to Regenerate Heavily Fouled Columns How to Replace a Column Frit How to Fill a Column Void
8.	HPLC Column Protection
		Evaluating the Performance of a Guard Column Minimal Dead Volume Maximizes Guard Column Efficiency Replace Guard Columns When... Guard Columns or Silica Saturator Columns
9.	Column Care and Storage
		Choosing Mobile Phases for Column Operation and Storage Mobile Phases for Column Storage Operational Guidelines Preventing Column Problems Repairing Column Problems
10.	Injection Solvent Effects
		The Problem The Explanation The Solution
11.	What Can You Do to Improve the Peak Shape of Bases and Acids?
		What Can You Do to Improve the Peak Shape of Bases? What Can You Do to Improve the Peak Shape of Acids?

MAC-MOD Analytical, Inc. -- info@mac-mod.com -- 1-800-441-7508