The analysis of highly polar analytes by conventional reversed-phase HPLC columns can be problematic if adequate retention can only be obtained using a mobile phase with less than 10% organic modifier. Such "high-aqueous" mobile phases fail to adequately wet the hydrophobic bonded phase, causing the phase to "collapse." Low efficiency, poor reproducibility, and slow column re-equilibration are the results.

HydroBond reversed-phase HPLC columns are ideal for method development requiring high or even 100% aqueous mobile phases. The use of polar functional groups in the bonded silane of these columns improves the "wettability" of the column, giving improved efficiency, retention and reproducibility.

Detailed information can be viewed on the website, downloaded in PDF format, and requested by US Mail.

For detailed information on HydroBond HPLC columns, select from the following supporting sources:

 

 

A complete description of the HydroBond stationary phases including particle size, pore size, surface area, carbon load, and pH range. Examples of the types of applications that are recommended for the HydroBond columns are shown, and a list of column part numbers and prices is included.
  

 

The cause of poor reproducibility when operating with highly aqueous mobile phases is described and suggestions are offered to eliminate it. This report also describes how stationary phases can be designed to avoid problems of reproducibility when using highly aqueous mobile phases and lists several brands of commercially available stationary phases that can be successfully used with even 100% aqueous mobile phases.
   

 

A difficult separation of seven chemically related compounds is successfully carried out with a mobile phase containing only 7% organic.
     
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