The ACE C18-Amide phase is a polar-embedded amide phase, with an extended spacer between the amide functional group and the silica surface which provides improved stability compared to other commercial amide-type phases and a C18 chain on the terminal end. 

The ACE C18-Amide column can be used with 100% aqueous mobile phases, and provides additional retention for hydrogen bond-donor analytes such as acids, phenolics, amines, amides, etc.  The C18-Amide phase provides alternate, orthogonal selectivity compared with other ACE phases such as C18, C18-AR, C18-PFP, SuperC18, and CN-ES, which can make method development much more effective.


  • Ideal column choice for method development
  • Alternative selectivity to C18 and C8 columns with polar molecules - especially for acids
  • Comparable retention to C18 columns for moderately polar and more hydrophobic analytes
  • Compatible with 100% aqueous mobile phases and provides increased retention for polar analytes
  • Stable using low and mid pH eluents
  • Low bleed for UV and LC/MS compatibility
  • High efficiency 2μm, 3μm, 5μm and 10μm particles for UHPLC, HPLC and preparative separations

Figure 1

ACE C18-Amide provides significantly increased retention and improved selectivity for polar protic analytes.

Figure 2

ACE C18-Amide can be used to adjust selectivity and “pull peaks apart”.

Care and Use Sheets Available on Request (email