Fused-Core particle technology was developed by Dr. Jack Kirkland to produce UHPLC columns that provide ultrahigh resolution and ultrafast separations without sacrificing column ruggedness and reliability. HALO columns generate significantly less back pressure compared to other UHPLC columns, putting less stress on the system and facilitating rugged reliable performance. It is this moderate back pressure of HALO columns that also permits them to be used with most conventional HPLC equipment for UHPLC performance. The combination of much lower backpressure and excellent efficiency at higher linear velocities permits the easy transfer of methods from UHPLC to HPLC, and vice versa.
HALO C18 & C8
Excellent performance for a broad range of analyte polarities. Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes.
HALO RP-Amide
Retention and selectivity due to both hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
HALO Peptide ES-C18
Ideal for ultrafast or high resolution separations of peptides and polypeptides ≤ 15 kDa. Enhanced stability at low pH via sterically protected ligands up to 90 degrees C.
HALO Phenyl-Hexyl
Separations occur due both to hydrophobic and π-π interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases.
HALO PFP
Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, π–π, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode with mobile phases containing > 80% acetonitrile. Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
Penta-HILIC
This unique bonded phase provides a highly polar stationary phase on which to perform HILIC separations. The high bonding density and polarity of the bonded phase minimize ion-exchange effects with residual silanols, thereby improving peak shape and lot-to-lot reproducibility.
HALO HILIC (silica)
Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
ES-CN
Provides strong dipole-dipole interactions with analytes and weak hydrophobic interactions. Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid equilibration with low bleed for high throughput LC-MS applications. Extra stable bonding with sterically protected ligand provides improved stability and performance compared to conventional CN phases, especially at low pH (1-4).