Hydrophilic Interaction Liquid Chromatography (HILIC) uses similar eluents to Reversed-Phase Liquid Chromatography (RPLC) and provides a suitable retention and separation alternative for hydrophilic, or polar to very polar analytes. The retention of these hydrophilic analytes (i.e., those species with a log P value of around zero or less) can be a challenge in RPLC, characterized by little or no retention. HILIC offers retention and alternative selectivity to RPLC for hydrophilic analytes without the use of additives such as ion-pairing reagents.
As a technique, HILIC has now been applied to almost all chromatography application areas and has been proven useful for clinical analysis, environmental analysis, food and beverage applications, the pharmaceutical industry and proteomics, where the retention and separation of polar to very polar analytes can still be challenging.
Our HILIC webinar provides an overview on how to develop robust HILIC methods with practical examples as well as useful advice for key operating parameters.
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