Abstract:
Choosing an HPLC column isn’t just about picking a C18 or CN phase and hoping for the best. In this dynamic and insightful webinar, Dr. Lee Polite of Axion Labs teams up with MAC-MOD to take you beyond surface-level decisions and into the real-world factors that determine successful separations. Whether you’re troubleshooting poor resolution, limited by system pressure, or scaling up methods for speed and reproducibility—this session will clarify what really matters.
We’ll explore how stationary phase chemistry interacts with column architecture—focusing on critical yet often overlooked variables like particle size, surface area, carbon load, and the fundamental differences between totally porous and superficially porous particles. Dr. Polite will demystify how column design affects efficiency, back pressure, and method robustness, drawing from decades of hands-on experience and real-world lab scenarios.
Topics will include:
- When superficially porous particles outperform—and when they don’t
- Why surface area and diffusion path length matter for resolution
- How to match particle size with system pressure limitations
- Practical trade-offs between efficiency, speed, and loadability
- And how to make smart column choices that actually work in your lab
Whether you’re using conventional HPLC or UHPLC systems, this webinar will equip you with the knowledge to choose columns more confidently and avoid the trial-and-error approach that wastes time and budget. Perfect for chromatographers in pharmaceutical, environmental, forensic, and academic labs—or anyone who wants to improve separations without overcomplicating their methods.