When developing a new LC or LC-MS separation, careful consideration of mobile phase composition is essential for optimizing peak shape, improving separation selectivity, and ensuring method robustness. For samples containing ionizable compounds, it is important to select and appropriate mobile phase pH to control the analyte ionization state, to achieve reliable retention times.  This white paper discusses the important aspects to consider when selecting mobile phase buffers to ensure the development of robust and reproducible LC separations.

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