Bonded Phase USP Listing Particle Size (µm) Pore Size (Å) pH Range Temp. Max °C
(low pH)
Temp. Max °C
(high pH)
Carbon Load (%) Surface Area (m2/g) Endcapped Buy
218TP L1

3
5
10

300

2-8 60 40

8

60-110

Yes Buy Now
238TP L1

5

300

2-8 60 40

4

60-110

Yes Buy Now
208TP L7

5

300

2-8 60 40

5

60-110

Yes Buy Now
214TP L26

5
10

300

2-8 60 40

3

60-110

Yes Buy Now
214ATP (C4) L26

5

300

2-8 60 40

3

60-110

Yes Buy Now
219TP L11

5
10

300

2-8 60 40

4

60-110

Yes Buy Now
201TP L1

3
5
10

300

2-8 60 40

8

70-90

No Buy Now
202TP L1

5

300

2-8 60 40

9

60-90

No Buy Now
218MS C18 L1

3
5
10

300

2-8 60 40

8

60-110

Yes Buy Now
238MS C18 L1

5

300

2-8 60 40

4

70

Yes Buy Now
208MS C8 L7

5

300

2-8 60 40

5

70

Yes Buy Now
214MS C4 L26

5
10

300

2-8 60 40

3

70-110

Yes Buy Now
238EV C18 L1

5
10

300

2-8 60 40

6

70-110

Yes Buy Now
219MS Diphenyl L11

5

300

2-8 60 40

4

70

Yes Buy Now

Additional Details

Vydac TP HPLC Columns

  • Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations
  • The 300 Å TP silica gives polypeptide molecules complete access to the interior of the silica pores
  • Vydac TP is known for long column lifetime and negligible phase leaching
  • Vydac TP provides reliable protein purifications, scalable from analytical to preparative scale
  • Vydac TP is referenced in a large number of patents and publications
  • Vydac 218TP and 238TP are used for small polypeptides of 4-5kDa, enzymatic digest fragments, natural and synthetic peptides and multi-ring compounds
  • Vydac 208TP is recommended for polypeptides of molecular weight 10-20kDa
  • Vydac 214TP is recommended for the analysis of glycoproteins, hemoglobin variants, histones, insulin variants and membrane proteins
  • Vydac 219TP is suitable for polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides and fusion proteins from inclusion bodies

Vydac MS Columns

  • Vydac MS columns have a proprietary surface treatment and bonding process that provides unique selectivity
  • A variety of reversed-phase options makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins300A pore size spherical silica
  • Excellent peak shape with little or no TFA
  • High protein recoveries make scale-up easy
  • The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.
  • Vydac 218MS and 238MS are used for simple enzymatic digests (< 12 proteins) or biomolecules (0-5kDa).
  • Vydac 208MS is ideal for biomolecules of molecular weight 5-10kDa.
  • Vydac 214MS has lower capacity than C18 or C8 and is suitable for hydrophobic proteins or when minimal organic solvent is required. It is recommended for biomolecules of > 10kDa molecular weight, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone.
  • Vydac 219MS has the lowest capacity and is highly selective for proteins with aromatic side chains

Vydac Everest HPLC Columns

  • Vydac Everest columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols.
  • Vydac Everest columns have increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.
  • Vydac Everest columns provide unique selectivity for hydrophilic and hydrophobic peptides
  • Vydac Everest columns provide high resolution for complex peptide digests
  • Vydac Everest columns provide excellent sensitivity with little or no TFA in mobile phase

Vydac Denali HPLC Columns

  • Vydac Denali (238DE) is a 120A C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes.
  • Vydac Denali HPLC columns is intended for applications for small molecule analyses of interest to pharmaceutical and environmental laboratories.
  • The general features of Vydac Denali include high retentivity, LC-MS of small molecules, and fully scalable from capillary to process